Docosahexaenoic Acid (DHA)
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چکیده
Handling & Preparation Instructions This purified fatty acid is liquid at room temperature (oil) and not soluble in water. The product is supplied sterile. It can be solubilized in undiluted serum, ethanol or DMSO. The solubility of Docosahexaenoic acid in ethanol is at least 100 mg/ml. Docosahexaenoic acid has a solubility of 1 mg/ml in a solution of 0.15 M Tris-HCL (pH 8.5). We strongly recommend using fresh preparation each day when aqueous buffers are involved. Essential fatty acids are also soluble in chloroform or ether. However those two organic solvents not recommended with the use of cells. After reconstitution, the product can be aliquoted and stored at -80°C. We recommend adding the essential fatty acids cocktail to the medium the day of use.
منابع مشابه
Docosahexaenoic acid level of breast milk of iranian women in low fish – consuming and high fish – consuming regions
Objective: Sufficient intakes of docosahexaenoic acid (DHA) via breast milk are required for optimizing visual and neural development at early stages of life. Little or no information is available on DHA intakes and levels found in breast milk in Iranian women and surrounding regions. In this study we measure the DHA in the breast milk of lactating Iranian women from low and high – fish-eating ...
متن کاملIn silico Screening and Evaluation of the Anticonvulsant Activity of Docosahexaenoic Acid-Like Molecules in Experimental Models of Seizures
Background: Resistance to antiepileptic drugs as well as intolerability in 20-30% of the patients raises demand for developing new drugs with improved efficacy and safety. Acceptable anticonvulsant activity, good tolerability, and inexpensiveness of docosahexaenoic acid (DHA), make it as a good candidate for designing and development of the new anticonvulsant medications. Methods: Ten DHA-based...
متن کاملLong-Term Effect of Docosahexaenoic Acid Feeding on Lipid Composition and Brain Fatty Acid-Binding Protein Expression in Rats.
Arachidonic (AA) and docosahexaenoic acid (DHA) brain accretion is essential for brain development. The impact of DHA-rich maternal diets on offspring brain fatty acid composition has previously been studied up to the weanling stage; however, there has been no follow-up at later stages. Here, we examine the impact of DHA-rich maternal and weaning diets on brain fatty acid composition at weaning...
متن کاملInhibitory and Dose-dependent effect of intracerebroventricular docosahexaenoic acid on clonic seizures induced by pentylenetetrazole in mice
Introduction: Epilepsy is one of the most common neurologic disorders. Pharmacoresistance and adverse effects of current antiepileptic drugs (AEDs) necessitate development of new drugs and strategies for treatment of epilepsy. Omega 3-Polyunsaturated fatty acids (ω3-PUFAs) are safe nutritional supplements that recently considered for treatment of epilepsy. Anticonvulsant effect of docosahexa...
متن کاملDocosahexaenoic acid and the preterm infant
Docosahexaenoic acid (DHA) is a long chain poly-unsaturated fatty acid (LCPUFA) that has a role in the cognitive and visual development, as well as in the immune function of newborns. Premature infants are typically deficient in DHA for several reasons, to include fetal accretion of DHA that typically occurs during the third trimester. These premature infants are reliant on enteral sources of D...
متن کاملHigh-fat meals rich in EPA plus DHA compared with DHA only have differential effects on postprandial lipemia and plasma 8-isoprostane F2a concentrations relative to a control high–oleic acid meal: a randomized controlled trial
Background: Eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) supplementation has beneficial cardiovascular effects, but postprandial influences of these individual fatty acids are
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